Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5193

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protocolProtocol

Immunostaining Helobdella (Leech) Embryos

David A. Weisblat1 and Dian-Han Kuo

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA

1Corresponding author (weisblat{at}berkeley.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

The purpose of this staining protocol is to detect the localization of specific antigens in the Helobdella (leech) embryo. Immunostaining protocols must be optimized for each antibody and embryonic stage. Here, as a starting point, we present a general-purpose immunostaining protocol. Immunostaining of the leech embryo can be performed on the intact embryo or on dissected parts. For post-gastrulation embryos (stages 9 and 10), if the area of interest is in the germinal plate, immunostaining can be performed on the dissected germinal plate. It can also be performed on the ventral nerve cords dissected from late-stage embryos or juveniles.


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Related Article

Helobdella (Leech): A Model for Developmental Studies
David A. Weisblat and Dian-Han Kuo
Cold Spring Harb Protoc 2009: 121. [Abstract] [Full Text]



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 Cold Spring Harb ProtocHome page
D. A. Weisblat and D.-H. Kuo
Helobdella (Leech): A Model for Developmental Studies
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 Cold Spring Harb ProtocHome page
D. A. Weisblat and D.-H. Kuo
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