Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5194

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In Situ Hybridization of Helobdella (Leech) Embryos

David A. Weisblat1 and Dian-Han Kuo

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA

1Corresponding author (weisblat{at}berkeley.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

The purpose of this protocol is to reveal the localization of transcripts in the Helobdella (leech) embryo. In situ hybridization protocols for Helobdella embryos are derived from those used for zebrafish and Xenopus. The protocols are different for early- and late-stage embryos (stages 1-8 and stages 9-11, respectively) due to a difference in tissue permeability.


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Related Article

Helobdella (Leech): A Model for Developmental Studies
David A. Weisblat and Dian-Han Kuo
Cold Spring Harb Protoc 2009: 121. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
D. A. Weisblat and D.-H. Kuo
Helobdella (Leech): A Model for Developmental Studies
Cold Spring Harb Protoc, April 1, 2009; 2009(4): pdb.emo121 - pdb.emo121.
[Abstract] [Full Text]

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Using BM Purple as an alternative AP color reaction substrate
Dian-Han Kuo
Cold Spring Harb Protoc, 9 Sep 2009 [Full text]