Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5198

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protocolProtocol

Quick Preparation of Genomic DNA from Drosophila

Audrey M. Huang, E. Jay Rehm, and Gerald M. Rubin

Adapted from Drosophila Protocols (eds. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

This protocol describes a standard miniprep for Drosophila melanogaster that requires very few flies and produces high-quality DNA. This method can also be used to isolate RNA when RNase-free conditions are utilized; an extra step must be taken to rid the sample of genomic DNA (e.g., RNase-free DNase digestion).


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 Cold Spring Harb ProtocHome page
A. M. Huang, E. J. Rehm, and G. M. Rubin
Recovery of DNA Sequences Flanking P-Element Insertions in Drosophila: Inverse PCR and Plasmid Rescue
Cold Spring Harb Protoc, April 1, 2009; 2009(4): pdb.prot5199 - pdb.prot5199.
[Abstract] [Full Text]