Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5158

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protocolProtocol

Whole-Mount In Situ Hybridization of Bichir (Polypterus) Embryos

Masaki Takeuchi1, Masataka Okabe2, and Shinichi Aizawa1,3

1 Laboratory for Vertebrate Body Plan, Center for Developmental Biology, Riken Kobe, Chuo-ku, Kobe 650-0047, Japan
2 Department of Anatomy, The Jikei University School of Medicine, Minatoku, Tokyo 105-8461, Japan

3Corresponding author (saizawa{at}cdb.riken.jp)

This protocol is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

Axis and germ-layer formations are central issues in vertebrate embryology. An intriguing question is how the mechanisms that existed in an ancestral vertebrate have been modified during vertebrate evolution. A major stream of vertebrates (Osteichthyes) evolved in two monophyletic lineages: the Sarcopterygii and Actinopterygii. There are many differences in the molecular and cellular mechanisms of embryogenesis between teleosts (actinopterygians) and amphibians (sarcopterygians), including the differences in bauplan (body plan). Polypterus diverged from all other actinopterygians ~400 million years ago (Mya) during the Devonian period, soon after the divarication of an ancestral bony fish into Actinopterygii and Sarcopterygii. Polypterus is thus uniquely well suited for studies assessing the ancestral state or bauplan of Osteichthyes and Actinopterygii, as well as the divergence of embryogenetic processes in teleosts and amphibians. This protocol describes a method for in situ hybridization in Polypterus embryos.


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Related Article

The Genus Polypterus (Bichirs): A Fish Group Diverged at the Stem of Ray-Finned Fishes (Actinopterygii)
Masaki Takeuchi, Masataka Okabe, and Shinichi Aizawa
Cold Spring Harb Protoc 2009: 117. [Abstract] [Full Text]

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Home page
 Cold Spring Harb ProtocHome page
M. Takeuchi, M. Okabe, and S. Aizawa
The Genus Polypterus (Bichirs): A Fish Group Diverged at the Stem of Ray-Finned Fishes (Actinopterygii)
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.emo117 - pdb.emo117.
[Abstract] [Full Text]