Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5206

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Fixation and Dissection of Embryos from the African Butterfly Bicyclus anynana

Paul M. Brakefield1, Patrícia Beldade, and Bas J. Zwaan

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes a procedure for the fixation and dissection of embryos from the African butterfly Bicyclus anynana. Such embryos can be used subsequently for in situ hybridization experiments or for immunohistochemistry analysis.


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 Cold Spring Harb ProtocHome page
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