Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5207

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brakefield, P. M.
Right arrow Articles by Zwaan, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brakefield, P. M.
Right arrow Articles by Zwaan, B. J.
Related Collections
Right arrow Laboratory Organisms, general
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrowRelated Article
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Dissection of Larval and Pupal Wings from the African Butterfly Bicyclus anynana

Paul M. Brakefield1, Patrícia Beldade, and Bas J. Zwaan

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes a procedure for the dissection of larval and pupal wings from the African butterfly Bicyclus anynana. Dissected pupal wings can be used subsequently for in situ hybridization or for immunohistochemistry. They can also be analyzed with stereomicroscopy to study scale maturation and pigment deposition.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
Cold Spring Harb Protoc 2009: 122. [Abstract] [Full Text]

Related Protocols

In Situ Hybridization of Embryos and Larval and Pupal Wings from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
Cold Spring Harb Protoc 2009: 5208. [Abstract] [Full Text]

Immunohistochemistry Staining of Wing Discs from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
Cold Spring Harb Protoc 2009: 5210. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.emo122 - pdb.emo122.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
In Situ Hybridization of Embryos and Larval and Pupal Wings from the African Butterfly Bicyclus anynana
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.prot5208 - pdb.prot5208.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
Immunohistochemistry Staining of Wing Discs from the African Butterfly Bicyclus anynana
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.prot5210 - pdb.prot5210.
[Abstract] [Full Text]