Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5208

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In Situ Hybridization of Embryos and Larval and Pupal Wings from the African Butterfly Bicyclus anynana

Paul M. Brakefield1, Patrícia Beldade, and Bas J. Zwaan

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

The modified in situ hybridization protocol described here can be used to localize RNA transcripts in developing tissues of the African butterfly Bicyclus anynana, including larval and pupal wings and embryos.


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The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.emo122 - pdb.emo122.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
Fixation and Dissection of Embryos from the African Butterfly Bicyclus anynana
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.prot5206 - pdb.prot5206.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
Dissection of Larval and Pupal Wings from the African Butterfly Bicyclus anynana
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.prot5207 - pdb.prot5207.
[Abstract] [Full Text]