Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5209

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Immunohistochemistry Staining of Embryos from the African Butterfly Bicyclus anynana

Paul M. Brakefield1, Patrícia Beldade, and Bas J. Zwaan

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes an immunohistochemical method for the detection of proteins in developing embryos of the African butterfly Bicyclus anynana. The embryos are stained with a primary antibody against a protein of interest, followed by a fluorescently labeled secondary antibody.


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Related Article

The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
Cold Spring Harb Protoc 2009: 122. [Abstract] [Full Text]

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Fixation and Dissection of Embryos from the African Butterfly Bicyclus anynana
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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Cold Spring Harb Protoc, May 1, 2009; 2009(5): 10.1101/pdb.emo122.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
Fixation and Dissection of Embryos from the African Butterfly Bicyclus anynana
Cold Spring Harb Protoc, May 1, 2009; 2009(5): 10.1101/pdb.prot5206.
[Abstract] [Full Text]