Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5213

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Constant Volume Respirometry in the African Butterfly Bicyclus anynana

Paul M. Brakefield1, Patrícia Beldade, and Bas J. Zwaan

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes the use of constant volume respirometry to measure CO2 production by the African butterfly Bicyclus anynana. The instrument used is the TR-2 system from Sable Systems International. In this setup, dry CO2-free air is sequentially pumped through 16 sealed chambers. The CO2 produced by the butterflies is subsequently measured with an infrared CO2 detector. CO2 production (µL/ h) by individual butterflies is an index of the resting metabolic rate (RMR). The RMR of each individual is corrected for weight, preferably after removal of water and fat mass, to allow comparison of the RMRs among individuals.


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The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
Cold Spring Harb Protoc 2009: 122. [Abstract] [Full Text]

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Home page
 Cold Spring Harb ProtocHome page
P. M. Brakefield, P. Beldade, and B. J. Zwaan
The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Cold Spring Harb Protoc, May 1, 2009; 2009(5): pdb.emo122 - pdb.emo122.
[Abstract] [Full Text]