Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot4981

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Phenotypic Analysis of Arabidopsis Mutants: Diaminobenzidine Stain for Hydrogen Peroxide

Joseph D. Clarke

Adapted from Arabidopsis: A Laboratory Manual (eds. Weigel and Glazebrook). CSHL Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

The oxidative burst characteristic of gene-for-gene resistance responses and early events in establishment of systemic acquired resistance can be detected using 3,3'-diaminobenzidine tetrahydrochloride (DAB), a stain for hydrogen peroxide. The presence of hydrogen peroxide, which is symptomatic of the hypersensitive response, causes polymerization of DAB, yielding a brown color. This protocol describes how to stain Arabidopsis tissue with DAB.


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