Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5224

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Fixation and Storage of Honeybee (Apis mellifera) Tissues

Peter K. Dearden1, Elizabeth J. Duncan, and Megan J. Wilson

Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand

1Corresponding author (peter.dearden{at}otago.ac.nz)


INTRODUCTION

This protocol describes the preparation and fixation of honeybee tissue. Honeybee embryos are very fragile and must be fixed overnight before they can be subjected to downstream applications such as in situ hybridization and immunohistochemistry.


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