Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5226

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In Situ Hybridization of Sectioned Honeybee (Apis mellifera) Tissues

Peter K. Dearden1, Elizabeth J. Duncan, and Megan J. Wilson

Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand

1Corresponding author (peter.dearden{at}otago.ac.nz)


INTRODUCTION

This protocol outlines how to carry out section in situ hybridization on honeybee larvae to detect expression of a gene of interest in individual cell types. It can also be modified to investigate gene expression in a sectioned honeybee brain. A digoxigenin-labeled probe is hybridized to fixed sections and the probe is detected by 4-nitro blue tetrazolium chloride (NBT)-5-bromo-4-chloro-3-indolyl-phosphate (BCIP) color development.


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P. K. Dearden, E. J. Duncan, and M. J. Wilson
The Honeybee Apis mellifera
CSH Protocols, June 1, 2009; 2009(6): pdb.emo123 - pdb.emo123.
[Abstract] [Full Text]