Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5227

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dearden, P. K.
Right arrow Articles by Wilson, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dearden, P. K.
Right arrow Articles by Wilson, M. J.
Related Collections
Right arrow Antibodies, general
Right arrow Cell Biology, general
Right arrow Protein Identification and Analysis
Right arrow Visualization
Right arrow Visualization of Proteins
Right arrow Genetics, general
Right arrow Laboratory Organisms, general
Right arrow Proteins and Proteomics, general
Right arrow Immunohistochemistry
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrow Immunostaining
Right arrow Immunostaining, general
Right arrowRelated Article
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Immunohistochemistry on Honeybee (Apis mellifera) Embryos

Peter K. Dearden1, Elizabeth J. Duncan, and Megan J. Wilson

Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand

1Corresponding author (peter.dearden{at}otago.ac.nz)


INTRODUCTION

This protocol describes the preparation and staining of honeybee embryos for immunohistochemistry. An antigen retrieval step is required in order to restore antigens that have become masked by the long fixation step required for honeybee embryos.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

The Honeybee Apis mellifera
Peter K. Dearden, Elizabeth J. Duncan, and Megan J. Wilson
Cold Spring Harb Protoc 2009: 123. [Abstract] [Full Text]

Related Protocol

Fixation and Storage of Honeybee (Apis mellifera) Tissues
Peter K. Dearden, Elizabeth J. Duncan, and Megan J. Wilson
Cold Spring Harb Protoc 2009: 5224. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
P. K. Dearden, E. J. Duncan, and M. J. Wilson
The Honeybee Apis mellifera
Cold Spring Harb Protoc, June 1, 2009; 2009(6): pdb.emo123 - pdb.emo123.
[Abstract] [Full Text]