Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5235

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Senghaas, N.
Right arrow Articles by Köster, R. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Senghaas, N.
Right arrow Articles by Köster, R. W.
Related Collections
Right arrow Cell Biology, general
Right arrow Analysis of Protein Expression in Cultured Cells
Right arrow Cell Culture
Right arrow Imaging/Microscopy, general
Right arrow Laboratory Organisms, general
Right arrow Zebrafish
Right arrow Proteins and Proteomics, general
Right arrow Immunohistochemistry
Right arrow Immunostaining
Right arrow Immunostaining Cells
Right arrow DNA Delivery/Gene Transfer
Right arrow Protein Classification and Structure Prediction
Right arrow DNA Delivery/Gene Transfer, general
Right arrow Non-Viral Methods
Right arrow Cell Imaging
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

Culturing and Transfecting Zebrafish PAC2 Fibroblast Cells

Niklas Senghaas1 and Reinhard W. Köster

Helmholtz Zentrum München, Institute of Developmental Genetics, D-85764 Munich-Neuherberg, Germany

1Corresponding author (niklas.senghaas{at}helmholtz-muenchen.de)


INTRODUCTION

The use of zebrafish (Danio rerio) as a model organism has seen rapid growth in recent years. Numerous new techniques have emerged for zebrafish, and many protocols used in other model organisms have been adapted to fit the special needs of this species. Zebrafish cell lines, however, have not been studied to a great extent, and their applications remain limited. The PAC2 fibroblast line, isolated from 24-h post-fertilization zebrafish embryos, is one of the few available lines. Here, we provide a basic set of methods for maintenance and handling of PAC2 cells, as well as general procedures for transfection, immunocytochemistry, and protein extraction.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Protocol

In Vivo Time-Lapse Imaging of Zebrafish Embryonic Development
Martin Distel and Reinhard W. Köster
CSH Protocols 2007: 4816. [Abstract] [Full Text]