Protocol

Stable Isotope and Elemental Analysis in Ants

¹ School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA
² Department of Biology, Linfield College, McMinnville, OR 97128, USA

³Corresponding author (csmith38{at}asu.edu)

INTRODUCTION

Over the past 20 yr, the use of stable isotopes to infer feeding ecology and the examination of how energetic and elemental exchanges are affected by and affect life (ecological stoichiometry) have gained momentum. The ecological diversity of ants makes them interesting models to explore dietary ecology and their role in food webs. Moreover, their ecological dominance in most habitats facilitates sampling. The protocol described here will produce samples adequate for submission to most labs that specialize in high-throughput analysis of stable isotopes; one should check with any particular lab for specific submission instructions. Note, however, that this protocol is designed specifically for the quantification of the natural abundance of stable isotopes; it does not cover the preparation of trace samples.