Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5244

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General Dissection of Female Ant Reproductive System and Brain

Adam Dolezal1,3 and Colin Brent2

1 School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA
2 USDA Arid-Land Agricultural Research Center, Maricopa, AZ 85238, USA

3Corresponding author (adam.dolezal{at}asu.edu)


INTRODUCTION

Dissection of the reproductive system of ant workers and queens can be useful for answering many questions. Observations of ovarian status in both female castes can be used to identify relationships between other factors and the ovaries, determine whether an individual has laid eggs, and, with more advanced molecular techniques, identify the end product of the oocyte in question (e.g., trophic egg, viable egg, nonviable egg). In addition, dissection of queens allows for observation of the spermatheca (the sperm storage organ), and thus identification of whether an individual has mated. Sperm can also be sampled for genetic analyses. In this protocol, we describe the dissection of the female ant reproductive system. We also discuss dissection of the corpora allata glands, where juvenile hormone is produced.


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Ants (Formicidae): Models for Social Complexity
Chris R. Smith, Adam Dolezal, Dorit Eliyahu, C. Tate Holbrook, and Jürgen Gadau
Cold Spring Harb Protoc 2009: 125. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
C. R. Smith, A. Dolezal, D. Eliyahu, C. T. Holbrook, and J. Gadau
Ants (Formicidae): Models for Social Complexity
Cold Spring Harb Protoc, July 1, 2009; 2009(7): pdb.emo125 - pdb.emo125.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
C. Brent and A. Dolezal
Radiochemical Assay of Juvenile Hormone Biosynthesis Rate in Ants
Cold Spring Harb Protoc, July 1, 2009; 2009(7): pdb.prot5248 - pdb.prot5248.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
A. Khila and E. Abouheif
In Situ Hybridization on Ant Ovaries and Embryos
Cold Spring Harb Protoc, July 1, 2009; 2009(7): pdb.prot5250 - pdb.prot5250.
[Abstract] [Full Text]