Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5247

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Ant Ecdysteroid Extraction and Radioimmunoassay

Colin Brent1 and Adam Dolezal2,3

1 USDA Arid-Land Agricultural Research Center, Maricopa, AZ 85238, USA
2 School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA

3Corresponding author (adam.dolezal{at}asu.edu)


INTRODUCTION

Ecdysteroids are a group of steroid compounds present in many plant and invertebrate species. In arthropods, they function primarily as hormones involved in the regulation of molting. This protocol describes how to extract ecdysteroid hormones from ant specimens and subsequently quantify circulating levels of the hormone. The hormone can be extracted from hemolymph or from whole-body homogenates of insects and quantified by radioimmunoassay.


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Related Article

Ants (Formicidae): Models for Social Complexity
Chris R. Smith, Adam Dolezal, Dorit Eliyahu, C. Tate Holbrook, and Jürgen Gadau
Cold Spring Harb Protoc 2009: 125. [Abstract] [Full Text]

Related Protocol

Juvenile Hormone Extraction, Purification, and Quantification in Ants
Colin Brent and Adam Dolezal
Cold Spring Harb Protoc 2009: 5246. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
C. R. Smith, A. Dolezal, D. Eliyahu, C. T. Holbrook, and J. Gadau
Ants (Formicidae): Models for Social Complexity
Cold Spring Harb Protoc, July 1, 2009; 2009(7): pdb.emo125 - pdb.emo125.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
C. Brent and A. Dolezal
Juvenile Hormone Extraction, Purification, and Quantification in Ants
Cold Spring Harb Protoc, July 1, 2009; 2009(7): pdb.prot5246 - pdb.prot5246.
[Abstract] [Full Text]