Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5248

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protocolProtocol

Radiochemical Assay of Juvenile Hormone Biosynthesis Rate in Ants

Colin Brent1 and Adam Dolezal2,3

1 USDA Arid-Land Agricultural Research Center, Maricopa, AZ 85238, USA
2 School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA

3Corresponding author (adam.dolezal{at}asu.edu)


INTRODUCTION

Juvenile hormone (JH) is an important insect hormone known to have many effects on development, reproduction, and behavior in both solitary and social insects. This protocol describes how to quantify in vitro biosynthesis rates from excised corpora allata (CA), the glands responsible for JH production. Excised glands are incubated with radiolabeled methionine, resulting in the production of radiolabeled JH III, which can then be quantified. This protocol is most useful when quantification of the activity of the glands, rather than circulating JH levels, is desired. Its difficulties lie primarily in the ability to remove and handle the glands correctly.


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Ants (Formicidae): Models for Social Complexity
Chris R. Smith, Adam Dolezal, Dorit Eliyahu, C. Tate Holbrook, and Jürgen Gadau
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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
C. R. Smith, A. Dolezal, D. Eliyahu, C. T. Holbrook, and J. Gadau
Ants (Formicidae): Models for Social Complexity
Cold Spring Harb Protoc, July 1, 2009; 2009(7): pdb.emo125 - pdb.emo125.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
A. Dolezal and C. Brent
General Dissection of Female Ant Reproductive System and Brain
Cold Spring Harb Protoc, July 1, 2009; 2009(7): pdb.prot5244 - pdb.prot5244.
[Abstract] [Full Text]