Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5261

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Dissection and Fixation of Large Milkweed Bug (Oncopeltus) Embryos

Paul Liu and Thomas C. Kaufman1

Department of Biology, Indiana University, Bloomington, IN 47405, USA

1Corresponding author (kaufman{at}indiana.edu)


INTRODUCTION

In this protocol, blastoderm- or germband-stage large milkweed bug (Oncopeltus) embryos are collected, fixed in paraformaldehyde, and dissected from the yolk mass. Germband-stage embryos are stained with SYTOX Green, a fluorescent DNA stain, to facilitate dissection.


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Related Article

Morphology and Husbandry of the Large Milkweed Bug, Oncopeltus fasciatus
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Cold Spring Harb Protoc 2009: 127. [Abstract] [Full Text]



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 Cold Spring Harb ProtocHome page
P. Liu and T. C. Kaufman
Morphology and Husbandry of the Large Milkweed Bug, Oncopeltus fasciatus
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 Cold Spring Harb ProtocHome page
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