Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5262

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In Situ Hybridization of Large Milkweed Bug (Oncopeltus) Tissues

Paul Liu and Thomas C. Kaufman1

Department of Biology, Indiana University, Bloomington, IN 47405, USA

1Corresponding author (kaufman{at}indiana.edu)


INTRODUCTION

In this protocol, double-label in situ hybridization of large milkweed bug (Oncopeltus) embryos is performed using labeled probes. Dual antibody staining is followed by detection with two different chromagens.


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Related Article

Morphology and Husbandry of the Large Milkweed Bug, Oncopeltus fasciatus
Paul Liu and Thomas C. Kaufman
Cold Spring Harb Protoc 2009: 127. [Abstract] [Full Text]

Related Protocol

Dissection and Fixation of Large Milkweed Bug (Oncopeltus) Embryos
Paul Liu and Thomas C. Kaufman
Cold Spring Harb Protoc 2009: 5261. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
P. Liu and T. C. Kaufman
Morphology and Husbandry of the Large Milkweed Bug, Oncopeltus fasciatus
Cold Spring Harb Protoc, August 1, 2009; 2009(8): pdb.emo127 - pdb.emo127.
[Abstract] [Full Text]