Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5263

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Axolotl (Ambystoma mexicanum) In Vitro Fertilization

Shahryar Khattak and Elly M. Tanaka1

Center for Regenerative Therapies, Dresden University of Technology, 01307 Dresden, Germany
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany

1Corresponding author (elly.tanaka{at}crt-dresden.de)


INTRODUCTION

Breeding of axolotl (Ambystoma mexicanum) can be accomplished by natural or artificial methods. Although natural matings are a very easy way of obtaining axolotl embryos, in vitro fertilization can have other advantages. For example, through in vitro fertilization, it is possible to know the exact time of fertilization. This protocol describes preparation of a sperm suspension and in vitro fertilization of axolotl eggs after injection of females with follicle stimulating hormone (FSH).


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Related Article

Ambystoma mexicanum, the Axolotl: A Versatile Amphibian Model for Regeneration, Development, and Evolution Studies
S. Randal Voss, Hans H. Epperlein, and Elly M. Tanaka
Cold Spring Harb Protoc 2009: 128. [Abstract] [Full Text]



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 Cold Spring Harb ProtocHome page
S. R. Voss, H. H. Epperlein, and E. M. Tanaka
Ambystoma mexicanum, the Axolotl: A Versatile Amphibian Model for Regeneration, Development, and Evolution Studies
Cold Spring Harb Protoc, August 1, 2009; 2009(8): pdb.emo128 - pdb.emo128.
[Abstract] [Full Text]