Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.ip69

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In Vivo Imaging of Mammalian Cells: Cell Engineering and Viability

Jason R. Swedlow, Paul D. Andrews, and Melpomeni Platani

Adapted from Live Cell Imaging (eds. Goldman and Spector). CSHL Press, Cold Spring Harbor, NY, USA, 2004.


INTRODUCTION

There are a number of instances in which the use of live cell imaging has provided critical insight into cellular and tissue function. It has therefore become a requisite analytical tool for use in cell biology, neurobiology, and developmental biology, as well as a routine method practiced in many biomedical research laboratories. The technical requirements for performing live cell imaging include a capacity to engineer appropriate cells for the imaging experiment and access to a digital image acquisition system. The availability of fluorescent protein (FP) technology allows the molecular specificity of fluorescent markers to be used in a genetically encoded manner. In this article, we describe techniques for the generation and maintenance of fluorescently labeled cells and their use in live cell imaging experiments.


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J. R. Swedlow, P. D. Andrews, and M. Platani
In Vivo Imaging of Mammalian Cells: Image Acquisition and Analysis
CSH Protocols, September 1, 2009; 2009(9): pdb.ip70 - pdb.ip70.
[Abstract] [Full Text]