Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5282

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In Situ Hybridization of Starlet Sea Anemone (Nematostella vectensis) Embryos, Larvae, and Polyps

Grigory Genikhovich and Ulrich Technau1

Department for Molecular Evolution and Development, Center for Organismal Systems Biology, Faculty of Life Sciences, University of Vienna, 1090 Wien, Austria

1Corresponding author (ulrich.technau{at}univie.ac.at).


INTRODUCTION

Whole-mount in situ hybridization is used to reveal the spatiotemporal expression pattern of a gene in tissue samples without sectioning the tissue. This protocol describes procedures for fixing starlet sea anemone (Nematostella vectensis) embryos, larvae, and polyps, hybridizing with a digoxigenin (DIG)-labeled RNA probe, and detecting the bound probe.


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Related Article

The Starlet Sea Anemone Nematostella vectensis: An Anthozoan Model Organism for Studies in Comparative Genomics and Functional Evolutionary Developmental Biology
Grigory Genikhovich and Ulrich Technau
Cold Spring Harb Protoc 2009: 129. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
G. Genikhovich and U. Technau
The Starlet Sea Anemone Nematostella vectensis: An Anthozoan Model Organism for Studies in Comparative Genomics and Functional Evolutionary Developmental Biology
Cold Spring Harb Protoc, September 1, 2009; 2009(9): pdb.emo129 - pdb.emo129.
[Abstract] [Full Text]