Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5283

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Anti-acetylated Tubulin Antibody Staining and Phalloidin Staining in the Starlet Sea Anemone Nematostella vectensis

Grigory Genikhovich and Ulrich Technau1

Department for Molecular Evolution and Development, Center for Organismal Systems Biology, Faculty of Life Sciences, University of Vienna, 1090 Wien, Austria

1Corresponding author (ulrich.technau{at}univie.ac.at).


INTRODUCTION

This protocol describes anti-acetylated tubulin antibody staining in the starlet sea anemone Nematostella vectensis. The antibody against acetylated tubulin mainly recognizes cilia in N. vectensis. In planula larvae, it not only stains the cilia covering the ectoderm, but also is a good marker for the apical tuft at the aboral pole. Variations of this protocol can be used for staining with other antibodies (Marlow et al. 2009). However, not all antigens are preserved by fixation in paraformaldehyde; thus, alternative fixation methods are also described. In addition, an optional step of phalloidin staining of fibrillar actin is included.


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Related Article

The Starlet Sea Anemone Nematostella vectensis: An Anthozoan Model Organism for Studies in Comparative Genomics and Functional Evolutionary Developmental Biology
Grigory Genikhovich and Ulrich Technau
CSH Protocols 2009: 129. [Abstract] [Full Text]

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Bromodeoxyuridine Labeling of S-Phase Nuclei in the Starlet Sea Anemone Nematostella vectensis
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G. Genikhovich and U. Technau
The Starlet Sea Anemone Nematostella vectensis: An Anthozoan Model Organism for Studies in Comparative Genomics and Functional Evolutionary Developmental Biology
CSH Protocols, September 1, 2009; 2009(9): pdb.emo129 - pdb.emo129.
[Abstract] [Full Text]