Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5284

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Bromodeoxyuridine Labeling of S-Phase Nuclei in the Starlet Sea Anemone Nematostella vectensis

Grigory Genikhovich and Ulrich Technau1

Department for Molecular Evolution and Development, Center for Organismal Systems Biology, Faculty of Life Sciences, University of Vienna, 1090 Wien, Austria

1Corresponding author (ulrich.technau{at}univie.ac.at).


INTRODUCTION

Bromodeoxyuridine (BrdU) is a thymidine analog that can be used to label cells in the S-phase of the cell cycle. BrdU penetrates the cell membrane and is rapidly integrated into the nucleotide pool, ready to be incorporated into DNA. A specific antibody against BrdU coupled to a fluorophore or an enzyme (alkaline phosphatase [AP] or horseradish peroxidase [HRP]) can detect BrdU incorporated into DNA. The method is simple and powerful, because the combination of pulse, continuous, and pulse-chase labeling allows deep insights into the cell-cycle dynamics and differentiation kinetics of a number of cycling and terminally differentiated cells. This protocol describes BrdU labeling of S-phase nuclei in the starlet sea anemone Nematostella vectensis.


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Related Article

The Starlet Sea Anemone Nematostella vectensis: An Anthozoan Model Organism for Studies in Comparative Genomics and Functional Evolutionary Developmental Biology
Grigory Genikhovich and Ulrich Technau
Cold Spring Harb Protoc 2009: 129. [Abstract] [Full Text]



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 Cold Spring Harb ProtocHome page
G. Genikhovich and U. Technau
The Starlet Sea Anemone Nematostella vectensis: An Anthozoan Model Organism for Studies in Comparative Genomics and Functional Evolutionary Developmental Biology
Cold Spring Harb Protoc, September 1, 2009; 2009(9): pdb.emo129 - pdb.emo129.
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 Cold Spring Harb ProtocHome page
G. Genikhovich and U. Technau
Anti-acetylated Tubulin Antibody Staining and Phalloidin Staining in the Starlet Sea Anemone Nematostella vectensis
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