Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.prot4954

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Phloroglucinol Stain for Lignin

Sarah Liljegren

Adapted from Arabidopsis: A Laboratory Manual (ed. Weigel and Glazebrook). CSHL Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

The lignin found in plant cell walls can be stained with phloroglucinol in the presence of alcohol and HCl, as described in this article. The cell walls will temporarily turn red.


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