Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.prot5351

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Püschel, B.
Right arrow Articles by Viebahn, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Püschel, B.
Right arrow Articles by Viebahn, C.
Related Collections
Right arrow Genetics, general
Right arrow Laboratory Organisms, general
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrow Evolution
Right arrow Evolutionary Development (Evo-Devo)
Right arrowRelated Article
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Dissection and Fixation of Rabbit Embryos

Bernd Püschel and Christoph Viebahn1

Department of Anatomy and Embryology, University of Göttingen, 37075 Göttingen, Germany

1Corresponding author (cviebah{at}gwdg.de).


INTRODUCTION

This protocol describes general methods for dissection and fixation of rabbit embryos. Depending on the investigation, embryos can be dissected either alive or after fixation. Some embryonic stages are surrounded by an envelope, the zona pellucida, which might have to be removed for some applications (e.g., immunohistochemistry). Fixation methods vary according to different experimental needs, such as structure preservation (morphology), antigen preservation (immunoreactivity), or mRNA accessibility (in situ hybridization).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Bernd Püschel, Nathalie Daniel, Eva Bitzer, Martin Blum, Jean-Paul Renard, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 139. [Abstract] [Full Text]

Related Protocol

Rabbit Mating and Embryo Isolation
Bernd Püschel and Christoph Viebahn
Cold Spring Harb Protoc 2010: 5350. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
B. Puschel, N. Daniel, E. Bitzer, M. Blum, J.-P. Renard, and C. Viebahn
The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.emo139.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel and C. Viebahn
Rabbit Mating and Embryo Isolation
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5350.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel, E. Bitzer, and C. Viebahn
Live Rabbit Embryo Culture
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5352.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel and C. Viebahn
Staining and Imaging of Live Rabbit Embryos
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5353.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel and C. Viebahn
Whole-Mount In Situ Immunofluorescence of Early Rabbit Embryos
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5354.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel, M. Blum, and C. Viebahn
Whole-Mount In Situ Hybridization of Early Rabbit Embryos
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5355.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel, E. Bitzer, M. Blum, and C. Viebahn
Mounting, Embedding, and Sectioning of Early Rabbit Embryos
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5356.
[Abstract] [Full Text]