Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.prot5352

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protocolProtocol

Live Rabbit Embryo Culture

Bernd Püschel1, Eva Bitzer2, and Christoph Viebahn1,3

1 Department of Anatomy and Embryology, University of Göttingen, 37075 Göttingen, Germany
2 Department of Zoology, University of Hohenheim, 70593 Stuttgart, Germany

3Corresponding author (cviebah{at}gwdg.de).


INTRODUCTION

This protocol outlines methods for culturing rabbit embryos. Such cultures can be used to study the impact of micromanipulation, transplantation, and the influence of signaling molecules on embryonic development. They can be performed on whole blastocysts submerged in culture medium (suspension culture) or on explanted parts of the embryo spread out on a semisolid agarose support (flat or ring culture). Cultures can be adapted to many desired growth conditions by varying the parameters, but culture length is limited to ~24 h because longer incubation times lead either to bursting of the blastocyst and subsequent rolling up of the embryonic disc or to inadequate growth, resulting in deformed embryos.


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Related Article

The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Bernd Püschel, Nathalie Daniel, Eva Bitzer, Martin Blum, Jean-Paul Renard, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 139. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
B. Puschel, N. Daniel, E. Bitzer, M. Blum, J.-P. Renard, and C. Viebahn
The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.emo139.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel and C. Viebahn
Rabbit Mating and Embryo Isolation
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5350.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel and C. Viebahn
Staining and Imaging of Live Rabbit Embryos
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5353.
[Abstract] [Full Text]