Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.prot5353

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Püschel, B.
Right arrow Articles by Viebahn, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Püschel, B.
Right arrow Articles by Viebahn, C.
Related Collections
Right arrow Cell Biology, general
Right arrow Visualization
Right arrow Visualization, general
Right arrow Genetics, general
Right arrow Imaging/Microscopy, general
Right arrow Labeling for Imaging
Right arrow Video Imaging / Time Lapse Imaging
Right arrow Laboratory Organisms, general
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrow Evolution
Right arrow Evolutionary Development (Evo-Devo)
Right arrow Cell Imaging
Right arrowRelated Article
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Staining and Imaging of Live Rabbit Embryos

Bernd Püschel and Christoph Viebahn1

Department of Anatomy and Embryology, University of Göttingen, 37075 Göttingen, Germany

1Corresponding author (cviebah{at}gwdg.de).


INTRODUCTION

This protocol describes methods for supravital labeling of cellular proliferation and movement in rabbit embryos as well as for imaging of live cell movements. The techniques use localized labeling of epiblast cells with DiI by injection or by using comprehensive nuclear staining of all cell layers before and during early gastrulation. Because rabbit embryos implant relatively late during embryonic development, gastrulation-stage embryos can be isolated by flushing them from the uterus. Moreover, the embryonic disc is flat and translucent, providing a direct uncompromised overview of gastrulation while the whole blastocyst is protected by the zona pellucida. These properties allow examination of whole blastocysts either under the impact of changing environmental conditions (e.g., medium additives, oxygen concentrations) or following mechanical manipulation of the embryonic disc using a needle penetrating the zona pellucida. Further manipulation (e.g., transplantation) can be performed after removing the embryo from the zona and explantation of the embryonic disc. These techniques allow embryonic growth centers to be identified and single-cell movements to be recorded.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Bernd Püschel, Nathalie Daniel, Eva Bitzer, Martin Blum, Jean-Paul Renard, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 139. [Abstract] [Full Text]

Related Protocols

Rabbit Mating and Embryo Isolation
Bernd Püschel and Christoph Viebahn
Cold Spring Harb Protoc 2010: 5350. [Abstract] [Full Text]

Dissection and Fixation of Rabbit Embryos
Bernd Püschel and Christoph Viebahn
Cold Spring Harb Protoc 2010: 5351. [Abstract] [Full Text]

Live Rabbit Embryo Culture
Bernd Püschel, Eva Bitzer, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 5352. [Abstract] [Full Text]

Whole-Mount In Situ Immunofluorescence of Early Rabbit Embryos
Bernd Püschel and Christoph Viebahn
Cold Spring Harb Protoc 2010: 5354. [Abstract] [Full Text]

Whole-Mount In Situ Hybridization of Early Rabbit Embryos
Bernd Püschel, Martin Blum, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 5355. [Abstract] [Full Text]

Mounting, Embedding, and Sectioning of Early Rabbit Embryos
Bernd Püschel, Eva Bitzer, Martin Blum, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 5356. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
B. Puschel, N. Daniel, E. Bitzer, M. Blum, J.-P. Renard, and C. Viebahn
The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.emo139.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel and C. Viebahn
Rabbit Mating and Embryo Isolation
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5350.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel, E. Bitzer, and C. Viebahn
Live Rabbit Embryo Culture
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5352.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
B. Puschel, E. Bitzer, M. Blum, and C. Viebahn
Mounting, Embedding, and Sectioning of Early Rabbit Embryos
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5356.
[Abstract] [Full Text]