Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.prot5355

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Whole-Mount In Situ Hybridization of Early Rabbit Embryos

Bernd Püschel1, Martin Blum2, and Christoph Viebahn1,3

1 Department of Anatomy and Embryology, University of Göttingen, 37075 Göttingen, Germany
2 Department of Zoology, University of Hohenheim, 70593 Stuttgart, Germany

3Corresponding author (cviebah{at}gwdg.de).


INTRODUCTION

This protocol describes whole-mount in situ hybridization of early rabbit embryos. The method allows specific mRNA expression to be localized with respect to position and intensity in predefined embryonic areas. Optimal resolution of mRNA distribution can be achieved by subsequently embedding in situ hybridized embryos for histological sectioning.


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 Cold Spring Harb ProtocHome page
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