Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.prot5357

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Embryo Transfer in Rabbits

Nathalie Daniel1 and Jean-Paul Renard

Unité Mixte de Recherche UMR 1198, Biologie du Développement et Reproduction, Institut National de la Recherche Agronomique, F-78350 Jouy-en-Josas, France

1Corresponding author (nat.daniel2{at}jouy.inra.fr).


INTRODUCTION

Because there is a particularly narrow time window for implantation in rabbits, embryo transfer in this species requires a high degree of synchrony between the physiological status of the recipient doe and the developmental stage of the embryo at the time of transfer. In some cases, however, especially after in vitro culture of reconstructed embryos or after an extended culture period (e.g. overnight), the pregnancy rate obtained after embryo transfer into asynchronous recipients can be increased relative to synchronous transfers. In addition to the surgical methods required, this protocol describes two scenarios for the preparation of recipient does: an asynchronous scenario for embryos produced by intracytoplasmic sperm injection (ICSI) and a synchronous scenario for the transfer of thawed morulae.


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Related Article

The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Bernd Püschel, Nathalie Daniel, Eva Bitzer, Martin Blum, Jean-Paul Renard, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 139. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
B. Puschel, N. Daniel, E. Bitzer, M. Blum, J.-P. Renard, and C. Viebahn
The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.emo139.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
N. Daniel and J.-P. Renard
Artificial Fertilization in Rabbits by Intracytoplasmic Sperm Injection (ICSI)
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5359.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
N. Daniel and J.-P. Renard
Cryopreservation of Rabbit Embryos
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5360.
[Abstract] [Full Text]