Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.prot5359

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Artificial Fertilization in Rabbits by Intracytoplasmic Sperm Injection (ICSI)

Nathalie Daniel1 and Jean-Paul Renard

Unité Mixte de Recherche UMR 1198, Biologie du Développement et Reproduction, Institut National de la Recherche Agronomique, F-78350 Jouy-en-Josas, France

1Corresponding author (nat.daniel2{at}jouy.inra.fr).


INTRODUCTION

Intracytoplasmic sperm injection (ICSI) consists of the injection, under microscopic observation, of a single spermatozoon directly into the cytoplasm of an oocyte using an injection capillary. This technique is used commonly in human medicine in cases of infertility. The advantage of ICSI is that the technique requires only one spermatozoon per oocyte for fertilization. Spermatozoa used for ICSI do not require high motility; ICSI works as long as the sperm nucleus is genetically intact. This protocol describes methods for performing ICSI in rabbits using either fresh or frozen/thawed sperm.


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Related Article

The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Bernd Püschel, Nathalie Daniel, Eva Bitzer, Martin Blum, Jean-Paul Renard, and Christoph Viebahn
Cold Spring Harb Protoc 2010: 139. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
B. Puschel, N. Daniel, E. Bitzer, M. Blum, J.-P. Renard, and C. Viebahn
The Rabbit (Oryctolagus cuniculus): A Model for Mammalian Reproduction and Early Embryology
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.emo139.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
N. Daniel and J.-P. Renard
Embryo Transfer in Rabbits
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5357.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
N. Daniel and J.-P. Renard
Artificial Insemination in Rabbits
Cold Spring Harb Protoc, January 1, 2010; 2010(1): 10.1101/pdb.prot5358.
[Abstract] [Full Text]