Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.ip78

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Calculating Linear Flow Velocity from Volumetric Flow Rate

Helena Hedlund

Adapted from Purifying Proteins for Proteomics (ed. Simpson). CSHL Press, Cold Spring Harbor, NY, USA, 2004.


INTRODUCTION

In chromatography, flow rates should be standardized for columns of different dimensions by expressing the flow rate as linear flow velocity (cm/h). This is defined as the volumetric flow rate (cm3/h) per unit cross-sectional area (cm2) of a given column. However, flow rates are usually measured as volumetric flow rates (mL/min). It is therefore necessary to be able to convert between the units, which can be done using the equation described here.


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Home page
 Cold Spring Harb ProtocHome page
J. Mohammad
Purification of Proteins by Chromatofocusing
Cold Spring Harb Protoc, February 1, 2010; 2010(2): 10.1101/pdb.prot5377.
[Abstract] [Full Text]