Cite as: Cold Spring Harb. Protoc.; 2010; doi:10.1101/pdb.top67

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mohammad, J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Mohammad, J.
Related Collections
Right arrow Protein Identification and Analysis
Right arrow Proteins and Proteomics, general
Right arrow Chromatography
Right arrow Chromatography, general
Right arrow Chromatofocusing
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

topic_introductionTopic Introduction

Chromatofocusing

Jamil Mohammad

Adapted from Purifying Proteins for Proteomics: A Laboratory Manual (ed. Simpson). CSHL Press, Cold Spring Harbor, NY, USA, 2004.


INTRODUCTION

Chromatofocusing separates proteins on the basis of differences in their isoelectric point (pI). The stationary phase (matrix) is usually a weak anion exchanger in which the functional groups are amines, for example, a polybuffer exchanger (PBE 118 and PBE 94) or Mono P column. The eluent is a buffer containing a large number of buffering species that together give a uniform buffering capacity over a broad pH range.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
J. Mohammad
Preparation of the Gel and Column for Chromatofocusing
Cold Spring Harb Protoc, February 1, 2010; 2010(2): 10.1101/pdb.prot5375.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. Mohammad
Preparation of the Sample for Chromatofocusing: Buffer Exchange and Desalting
Cold Spring Harb Protoc, February 1, 2010; 2010(2): 10.1101/pdb.prot5376.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. Mohammad
Purification of Proteins by Chromatofocusing
Cold Spring Harb Protoc, February 1, 2010; 2010(2): 10.1101/pdb.prot5377.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. Mohammad
Removal of Elution Buffer from Protein Sample Fractions after Chromatofocusing
Cold Spring Harb Protoc, February 1, 2010; 2010(2): 10.1101/pdb.prot5378.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. Mohammad
Cleaning, Regeneration, and Storage of Chromatofocusing Columns
Cold Spring Harb Protoc, February 1, 2010; 2010(2): 10.1101/pdb.prot5379.
[Abstract] [Full Text]