Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4990

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Decalcifying Tissues for Paraffin Embedding

Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller

This protocol was adapted from "Preparation of Cells and Tissues for Fluorescence Microscopy," Chapter 4, in Basic Methods in Microscopy (eds. Spector and Goldman). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2006.


INTRODUCTION

Paraffin sections of bone usually require a decalcification step after fixation before sectioning. This protocol describes a method for decalcifying fixed tissue.


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A. H. Fischer, K. A. Jacobson, J. Rose, and R. Zeller
Paraffin Embedding Tissue Samples for Sectioning
CSH Protocols, May 1, 2008; 2008(6): pdb.prot4989 - pdb.prot4989.
[Abstract] [Full Text]