Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4478

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Reduction of Contamination of Epithelial Cultures by Fibroblasts

Jonathan C.R. Jones

This protocol was adapted from "Culture of Epithelial Cells," Chapter 5, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998.


INTRODUCTION

This protocol describes a method to reduce the numbers of fibroblasts from adherent populations of epithelial cells by using differential trypsinization.


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