Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5029

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Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila

Tony D. Southall and Andrea H. Brand1

The Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom

1Corresponding author (ahb{at}mole.bio.cam.ac.uk)


INTRODUCTION

The GAL4 system is a method for ectopic gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. This protocol describes the generation of driver and reporter lines for use with the GAL4 system in Drosophila. A promoter-GAL4 fusion is constructed using a P-element transformable vector, and a GAL4-responsive target gene is created via generation of an upstream activation sequence (UAS)-reporter construct. An alternative strategy for integration using the phiC31 system is also provided. Transformant lines are generated using standard procedures for microinjection.


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T. D. Southall, D. A. Elliott, and A. H. Brand
The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila
CSH Protocols, July 1, 2008; 2008(8): pdb.top49 - pdb.top49.
[Abstract] [Full Text]