Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4107

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Laser Capture Microdissection (LCM): Preparation and Sectioning of Frozen Tissue Blocks and Purification of RNA from Isolated Cells

Motoko Morimoto, Masahiro Morimoto, Jeanette Whitmire, Robert A. Star, Joseph F. Urban, Jr., and William C. Gause

This protocol was adapted from "Laser Capture Microdissection," contributed by Motoko Morimoto, Masahiro Morimoto, Jeannette Whitmire, Robert A. Star, Joseph F. Urban, Jr., and William C. Gause, Chapter 11, in PCR Primer, 2nd edition (eds. Dieffenbach and Dveksler). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 100 words of the full text of this article appear below.


INTRODUCTION

Laser capture microdissection (LCM) uses an infrared laser, incorporated within a standard microscope, to isolate specific cells or tissues from samples mounted on microscope slides. The samples are viewed through a thermoplastic film that is attached to a microcentrifuge tube lid. Localized heat, caused by the application of a laser pulse, fuses the membrane to the cells of interest, which can then be harvested for further analysis. RNA and proteins can be purified from the isolated cells, allowing detailed analysis of gene expression. This protocol is divided into three stages: the preparation of tissue samples for LCM; staining of slides . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


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