Protocol

Using Acetone and Trichloroacetic Acid to Concentrate Proteins and Remove Interfering Substances

This protocol was adapted from "Using Acetone and TCA to Concentrate Proteins and Remove Interfering Substances," contributed by Joint ProteomicS Laboratory (JPSL) of the Ludwig Institute for Cancer Research and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia, Appendix 2, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

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INTRODUCTION

Organic solvents such as acetone/TCA are useful for precipitating proteins and removing salts and detergents prior to protein analysis. This protocol was first described by Smith et al. (1985) to prepare protein samples for analysis using bicinchoninic acid.

MATERIALS

Reagents

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Equipment

METHOD