Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3825

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protocolProtocol

Purification of PCR Products in Preparation for Cloning

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

The residual enzymatic activity of thermostable DNA polymerases that survive the rigors of PCR can compromise subsequent enzymatic reactions. This protocol describes how to use proteinase K to destroy thermostable enzymes and to purify amplified DNA in preparation for cloning.


MATERIALS

recipe caution Ammonium acetate (10 M)

Optional, please see Step 3.

Amplified DNA from polymerase chain reactions

caution Chloroform

Optional, please see Step 7.

Ethanol

Optional, please . . . [Full Text of this Article]


METHOD


TROUBLESHOOTING


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P. D'Arpa
Strategies for Cloning PCR Products
Cold Spring Harb Protoc, August 1, 2009; 2009(8): pdb.ip68 - pdb.ip68.
[Abstract] [Full Text]