Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3968

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Related Collections
Right arrow Virus
Right arrow Molecular Biology, general
Right arrow Bacteriophage lambda
Right arrow Libraries
Right arrow Libraries, general
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Purification of Bacteriophage {lambda} Particles by Isopycnic Centrifugation through CsCl Gradients

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Isopycnic centrifugation through CsCl gradients is used to prepare infectious bacteriophage {lambda} particles of the highest purity that are essentially free of contaminating bacterial nucleic acids.


MATERIALS

CsCl (solid)

Ethanol

Optional, please see Step 5.

recipe SM

Discard each aliquot after use to minimize contamination.

Suspension of bacteriophage {lambda} particles

Prepared as described in Precipitation of Bacteriophage {lambda} Particles from Large-scale Lysates.


METHOD

1. Use a high quality (molecular biology grade) of solid CsCl to prepare three solutions of different densities by adding solid CsCl to SM, as indicated below. Store the . . . [Full Text of this Article]


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?