Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4026

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sambrook, J.
Right arrow Articles by Russell, D. W.
Related Collections
Right arrow Molecular Biology, general
Right arrow DNA Purification
Right arrow Electrophoresis, general
Right arrow Electrophoresis of Nucleic Acids
Right arrow Electrophoresis of Nucleic Acids, general
Right arrow Electrophoresis of DNA
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

Recovery of DNA from Low-melting-temperature Agarose Gels: Enzymatic Digestion with Agarase

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

A fragment of gel containing a band of DNA is excised and digested with agarase, which hydrolyzes the polymer to disaccharide subunits. The released DNA is then purified by phenol extraction and ethanol precipitation. The method works well for DNAs ranging in size from <5 kb to >20 kb.


MATERIALS

Agarase

DNA sample

recipe DNA staining solution

For a discussion of staining agarose gels, please see Detection of . . . [Full Text of this Article]


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?