Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4093

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PCR Amplification of Highly GC-Rich Regions

Lise Lotte Hansen and Just Justesen

This protocol was adapted from "PCR Amplification of Highly GC-Rich Regions," contributed by Lise Lotte Hansen and Just Justesen, Chapter 5, in PCR Primer, 2nd edition (eds. Dieffenbach and Dveksler). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

The following procedure details the establishment of an amplification procedure for GC-rich sequences. The DNA fragments of interest are amplified in the presence of either 5% DMSO, 1 M betaine, 2 M betaine, 1 M betaine, and 5% DMSO; 2 M betaine and 5% DMSO; 0.4 M tetramethylene sulfone; or without any of the enhancers.


MATERIALS

Reagents

Betaine (Sigma-Aldrich)

recipe dNTP solution (containing all four . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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