Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4214

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Preparation of a Prepacked IMAC Column

Lars Haneskog

This protocol was adapted from "Immobilized Metal-Ion Affinity Chromatography," contributed by Lars Haneskog, Chapter 12, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

The first 15% of the full text of this article appears below.


INTRODUCTION

Purification of histidine-tagged proteins on a milligram scale can be performed conveniently on a small (1- to 5-ml) immobilized metal-ion chromatography (IMAC) column using a syringe to load the sample, wash the column, and elute the protein. Columns can be connected in series, . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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