Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4353

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Setting Up Microdrop Cultures

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Recovery and In Vitro Culture of Preimplantation-Stage Embryos," Chapter 4, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes how to set up microdrop cultures to produce embryos which can then be used for making chimeras. The microdrop culture should be set up several hours to 1 day before the experiment to permit temperature and gas equilibration.


MATERIALS

Reagents

Appropriate . . . [Full Text of this Article]

Equipment


METHOD


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