Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4354

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Making Pipettes From Hard Glass Capillaries

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Recovery and In Vitro Culture of Preimplantation-Stage Embryos," Chapter 4, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

Pipettes are used for manipulating and transferring mouse embryos. This protocol describes a method for making pipettes from hard glass capillaries.


MATERIALS

Equipment

Bunsen/alcohol burner (small) or microburner

Glass test tubes with metal caps (BDH)

Hard glass capillary tubing (1.5-mm external diameter) or narrow part of glass Pasteur pipette cut off with a diamond-point pencil . . . [Full Text of this Article]


METHOD


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