Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4359

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Collecting Two-Cell- to Compacted Morula-Stage Embryos

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Recovery and In Vitro Culture of Preimplantation-Stage Embryos," Chapter 4, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for collecting two-cell- to compacted morula-stage embryos. Such embryos can then be used for microinjection.


MATERIALS

Reagents

Ethanol, 70%

recipe M2 medium at room temperature

Pregnant female mice (20-60 hours post coitum) sacrificed humanely (See Quick and Humane Sacrifice of a Mouse by Cervical Dislocation)

Equipment

Embryo-handling pipette consisting of mouth or hand-held pipette assembly and pulled capillary (see Making Pipettes from Hard Glass Capillaries and Preparing Siliconized Pipettes)

Flushing needle (either a 30- or 32-gauge hypodermic needle [end cut and/or ground to a blunt tip on an abrasive stone . . . [Full Text of this Article]


METHOD


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