Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4442

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Concentrating Hypotrichs

Carolyn L. Jahn

This protocol was adapted from "Culture and Manipulation of Hypotrichous Ciliated Protozoa," Chapter 17, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for concentrating either freshwater or marine hypotrichs for refeeding or analysis. A concentrating apparatus is constructed using a plastic "snap-close" container fitted with a Nitex filter of appropriate pore size.


MATERIALS

Reagents

Culture medium

Hypotrich culture to be concentrated

Equipment

Cheesecloth (Optional, see Step 4)

Collection tray (dishpan or other tray larger than plastic box to be converted to frame)

Nitex filter (Tetco), 45-55 . . . [Full Text of this Article]


METHOD


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