Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3588

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Autoradiography and Reading of Sequencing Gels

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol describes fixation of a sequencing gel and processing in preparation for autoradiography.


MATERIALS

recipe caution Radioactive ink

Reusable alternatives to radioactive ink are chemiluminescent markers available from Stratagene (Glogos). The markers can be used multiple times and should be exposed to fluorescent light just prior to a new round of autoradiography.

recipe Sequencing gel-fixing solution


METHOD

1. At the end of the electrophoretic run (Loading and Running DNA Sequencing Gels), turn off the power and disconnect the sequencing apparatus from the power pack. Dispose of the electrophoresis buffer and then remove the gel mold from the apparatus.

2. Lay the gel . . . [Full Text of this Article]


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