Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3807

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protocolProtocol

Preparation of Denaturing Polyacrylamide Gels Containing Formamide

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

The inclusion of formamide in sequencing gels eliminates secondary structure in the DNA during electrophoresis. Formamide gels are particularly useful and almost a necessity when sequencing DNA templates with a G/C content >55%. This protocol requires all of the reagents listed in Preparation of Denaturing Polyacrylamide Gels.


MATERIALS

recipe caution Formamide (100%)


METHOD

. . . [Full Text of this Article]


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